Prof. Leonid Chernin

Research Interests

  • Bacterial biocontrol agents of fungal and bacterial plant pathogens;
  • Genetics, regulation and mechanisms of the bacteria biocontrol activity;
  • Cell to cell communication (quorum-sensing) in plant-associated bacteria;
  • Genetic manipulation of plant growth promoting bacteria and plants to enhance biocontrol of phytopathogens;,
  • Oncogenicity of Agrobacterium tumefaciens and its control


See also: Leonid Chernin


Selected Publications


Alikhanyan, S.I., Akifyev, A.P. and Chernin, L.S. (1985) General Genetics (textbook). “High School” Publ., Moscow, 446 pp. (Rus.)

Chernin, L.S. (1990) First steps in future: plant genetic engineering. “Agropromizdat” Publ. Moscow, 255 pp. (Rus.)

Reviews/chapters in books and journals:

Chernin, L.S. (1977) Genetic engineering. In: “Great Medical Encyclopedia”, v. 5. “Medicine" Publ., Moscow (Rus.).

Kruger, D.H., and Chernin, L.S. (1979) Barrieren des Gentransfers werden uberwunden. Medizin Actuell 6:262-263.

Chernin, L.S. and Mikoyan, V.S. (1981) Effects of plasmid on chromosome metabolism in bacteria. Plasmid 6:119-140.

Chernin, L.S. (1982) Bacteria. In: “Annals of Sciences and Techniques. General problems of biology”, v. 1 “Genetic collections of microorganisms” (S. G. Inge-Vechtomov, ed.), Inst. Sci. Technical Inform. Publ. House, Moscow, pp. 107-147 (Rus.)

Chernin, L.S. (1983) Plasmids of phytopathogenic bacteria in the processes of oncogenesis and differentiation of plant cells (review). In: “Pathological hyperplasias in plants” (N. M. Emanuel, ed.), Institute of Chemical Physics Publishing House, Chernogolovka, Moscow Region, pp. 114-138 (Rus.).

Chernin, L.S. and Avdienko, I.D. (1985) Plasmid genes of phytohormones and their role in plant oncogenesis (a review) Molekularnaya Biologiya (Molecular Biology, Engl. Transl.) 19:869-889.

Kruger, D.H., Reuter, M., Chernin, L.S., et al. (1990) Biologishe Functioned der Restriktionsendonukleasen. Biol.Zent.Bl. 109: 257-266.

Chernin, L.S. (1994) Molecular Genetics. In “Encyclopedia of Genetics” (Shestakov, S.V., ed.). “Russian Encyclopedia” Publ., Moscow (Ru.).

Ovadis, M., Chernin L., Tzfira, T., Canaan V., Aharoni, A., Sakar, D. and Vainstein, A. (1998) Transformation of tobacco and aspen plants with the ita locus of an IncQ plasmid confers resistance to Agrobacterium tumefaciens infection, pp. 533-536. In: Plant Biotechnology and in vitro Biology. (A. Altman, S. Izhar, M. Ziv eds.), Kluwer Acad. Publish., Netherlands.,

Chernin, L. and Chet, I. (2002). Microbial enzymes in biocontrol of plant pathogens and pests, pp. 171-225. In: Enzymes in the Environment: Activity, Ecology, and Applications. (R. Burns, and R. Dick, eds.), Marcel Dekker, Inc., New York.

Chet, I. and Chernin, L. (2002). Biocontrol, Microbial agents in soil, pp. 450-465. In: Encyclopedia of Environmental Microbiology (G. Bitton, ed), John Willey & Sons Inc., New York.

Viterbo, A., Ramot, O., Chernin, L. and Chet, I. (2002). Significance of lytic enzymes from Trichoderma spp. in the biocontrol of fungal plant pathogens. Antonie van Leeuwenhoek 81: 549-556.,

Rice, S.A., Kjelleberg, S., Givskov, M., De Boer, W. and Chernin, L. (2004). Detection of bacterial homoserine lactone quorum sensing signals. In: Molecular Microbial Ecology Manual, 2nd edition. Kowalchuk, GA, de Bruijn FJ, Head IM, Akkermans ADL, van Elsas JD (eds) Chapter 8.04: 1629-1649. Kluwer Academic Publishers, Dordrecht, The Netherlands.,

Chernin, L. (2011) Quorum-sensing signals as mediators of PGPRs' beneficial traits. In: Bacteria in Agrobiology, vol. 3: Plant Nutrient Management. (Maheshwari, D.K., ed.). Chapter 9:, 209-236. Springer-Verlag Berlin Heidelberg.

Chernin, L.S. and Glick, B.R. (2012) The use of ACC deaminase to increase the tolerance of plants to various phytopathogens. In: Bacteria in Agrobiology, vol. 4: Stress Management (Maheshwari, D.K., ed.). Chapter 14: 279-299. Springer-Verlag Berlin Heidelberg.

Chernin, L., Toklikishvili, N., Dandurishvili, N., Tediashvili, M. and Vainstein, A. (2013) Suppression of Crown Gall Disease by Rhizosphere Bacteria and Agrobacterium-Specific Bacteriophages, in Molecular Microbial Ecology of the Rhizosphere (de Bruijn, F.J., ed.): vol., 2, Ch. 57, pp. 607-613. John Wiley & Sons, Inc., Hoboken, NJ, USA.

Chernin, L., Toklikishvili, N., Ovadis, M. and Khmel, I. (2013) Quorum-Sensing Quenching by Volatile Organic Compounds Emitted by Rhizosphere Bacteria, in Molecular Microbial Ecology of the Rhizosphere (de Bruijn, F.J., ed.): vol. 2, Ch. 75, pp. 791-800. John Wiley & Sons, Inc., Hoboken, NJ, USA.

Helman, Y. and Chernin, L. (2015) Silencing the mob: disrupting quorum sensing as a means to fight plant disease. Mol Plant Pathol. 2015;16:316-329

Articles in refereed journals:

Undritsev, M.I., Rosen, V.B. and Chernin, L.S. (1964) Glucocorticoid hormones in the pathogenesis of experimental rheumatic fever. Questions of Rheumatism 1:7-16 (Rus.).

Rozen, V.B. and Chernin, L.S. (1965) Effects of different doses of glucocorticoids on growth of monolayer cultures of connective tissue. Fed Proc Transl Suppl. 24: 861-862.,

Goldfarb, D.M., Chernin, L.S., and Fomichev, Yu.K. (1969) Inhibition of nitrogen mustard after-effect by the factor produced by E. coli K12 fertile strains. Molec.Gen.Genet. 104:107-115.,

Goldfarb, D.M., Chernin, L.S., Goldberg, G.I. et al. (1970) Two kinds of activity promoted by culture filtrates of male strains of E. coli K12: inhibition of "nitrogen mustard after-affect" and stimulation of genetic recombination. Molec.Gen.Genet. 106:315-322.

Chernin, L.S., G.I. Goldberg, and Goldfarb, D.M. (1970) DNA synthesis in F cells treated with filtrates of male strains of E. coli K12. Molec.Gen. Genet. 108:376-377.,

Goldfarb, D.M., Goldberg, G.I., Chernin, L.S. et al. (1973) A protein produced by male strains of Escherichia coli K12 that increases the yield of recombinants in conjugation: its nature and mode of action. Molec. Gen. Genet. 120:211-226.

Goldfarb, D.M., Gukova, L.A., Chernin, L.S. et al. (1974) Rfs mutants of Esherichia coli HfrC defective in the production of the factor stimulating recombination in conjugation. Molec.Gen.Genet. 129: 295-310.

Chernin, L.S., Ovadis, M.I., and Goldfarb, D.M. (1978) F plasmid genes involved in the production of recombination-stimulating factor, control of sensitivity to some injurious agents, and chromosome replication in Escherichia coli K12 HfrC. J. Bacteriol. 133:33-,

Kruger, D., Chernin, L.S. Hansen, S., Rosenthal, H.A., and Goldfarb, D.M. (1978) Protection of foreign, DNA against host, controlled restriction in bacterial cells. I. Protection of F'plasmid DNA by preinfection with bacteriophages T3 or T7. Molec. Gen.Genet. 159:107-110.

Chernin, L.S., and Ovadis, M.I. (1980) Plasmid control of recombination in E. coli K12. Molec.Gen Genet. 179:399-407.

Kruger, D.H., Reuter, M., Schroeder, C., Glatman, L.I., and Chernin, L.S. (1983) Restriction of bacteriophage T3 and T7 ocr+ strains by the type II restriction endonuclease EcoRV. Mol. Gen. Genet. 190:349- 351.

Kruger, D.H., Hansen, S., and Chernin, L.S. (1983) Abortive infection of F-plasmid-containing Escherichia coli cells by bacterial virus T7 is determined by the right end of T7 gene 1. J. Virol. 46:293-296.

Chernin, L.S., Lobanok, E.V., Fomicheva, V.V., and Kartel, N.A. (1984) Crown gall-suppressive IncW R plasmids cause a decrease in auxin production in Agrobacterium, tumefaciens. Mol. Gen. Genet. 195:195-199.

Terentyev, M.A., Ovadis, M.I., and Chernin, L.S. (1986) Superoligomerization of plasmid pACYC184 in recBmutant of, Escherichia, coli K12 determined by plasmid R1drd-19. Molecular Biology (Molekularnaya Biologiya, Engl. Transl.) 20:1631-1637.

Chernin, L.S., Ismailov, Z.F., Haran, S. and Chet, I. (1995) Chitinolytic Enterobacter agglomeransantagonistic to fungal plant pathogens. Appl. Environ. Microbiol. 61:1720-1726.

Chernin, L.S., Brandis, A., Ismailov, Z. F. and Chet, I. (1996) Pyrrolnitrin production by an Enterobacter agglomerans strain with a broad spectrum of antagonistic activity towards fungal and bacterial phytopathogens. Curr. Microbiol. 32:1-5.

Chernin, L., De La Fuente, L., Sobolev V., Haran, S., Vorgias, C.E., Oppenheim, A.B. and Chet I. (1997) Molecular cloning, structure analysis, and expression in Escherichia coli of a chitinase from Enterobacter agglomerans. Appl. Environ. Microbiol. 63: 834-839.

Chernin, L.S., Gafni, A., Mozes-Koch, R., Gerson, U. and Sztejnberg A.. 1997. Chitinolytic activity of the acaropathogenic fungi Hirsutella thompsonii and Hirsutella necatrix. Can. J. Microbiol. 43: 440-446.

Pleban, S., Chernin, L. and Chet, I. (1997). Chitinolytic activity of endophytic strain of Bacillus cereus. Lett. Appl. Microbiol. 25: 284-288.

Khmel, I.A., Sorokina, T.A., Lemanova, N.B., Lipasova, V.A., Metlitski, O.Z., Burdeinaya, T.V. and Chernin L.S. (1998) Biological control of crown gall in grapevine and raspberry by two Pseudomonas strains with a wide spectrum of antagonistic activity. Biocont. Sci. Technol., 8: 45-57.

Chernin, L.S., Winson, M.K., Thompson, J.M., Haran, S., Bycroft, B. W., Chet, I., Williams, P. and Stewart, G. S. A. B. (1998) Chitinolytic activity in Chromobacterium violaceum: substrate analysis and regulation by quorum sensing. J. Bacteriol. 180: 4435-4441.

Khmel, I.A., Veselova, M.A., Metlitskaya, A.Z., Klein, S., Lipasova, V.A., Mayatskaya, A.V., Chernin, L.S.(2002). Synthesis of signaling N-acyl-homoserine-lactones participating in quorum sensing regulation in rhizospheric and soil-borne bacteria Pseudomonas and Xanthomonas. Russ. J. Genet. 38: 467-469.

Kamensky, M., Ovadis, M., Chet, I. and Chernin, L. (2003). Soil-borne strain IC14 of Serratia plymuthicawith multiple mechanisms of antifungal activity provides biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum diseases Soil Biol. Biochem. 35: 323-331.,

Zohar-Perez, C., Chernin, L., Chet, I. and. Nussinovitch, A (2003) Structure of dried cellular alginate matrix containing fillers provides extra protection for microorganisms against UVC radiation. Radiat. Res. 160: 198-204.

Veselova, M., Kholmeckaya, M., Klein, S., Voronina, E., Lipasova, V., Metlitskaya, A, . Mayatskaya, A.,, Lobanok, E., Khmel, I. and Chernin, L. (2003) Production of N-acyl homoserine lactone signal molecules in Gram-negative soil-borne and plant-associated bacteria. Folia Microbiol. 48: 794-798.

Ovadis, M., Liu, X., Gavriel, S., Ismailov, Z., Chet, I. and Chernin, L. (2004) The global regulator genes from biocontrol strain Serratia plymuthica IC1270: cloning, sequencing, and functional studies. J. Bacteriol. 186: 4986-4993.

De La Fuente, L., Thomashow L., Weller, D., Bajsa, N., Quagliotto, L., CherninL. and Arias, A. (2004) Pseudomonas fluorescens UP61 isolated from birdsfoot trefoil rhizosphere produces multiple antibiotics and exerts a broad spectrum of biocontrol activity. Eur. J. Plant Pathol. 110: 671-681.

Khmel, I.A., Ovadis, M.I., Mayatskaya, A.V., Veselovskii, A.M., Bass, I.A., Lipasova, V.A., Bolshoy, A., Chet, I. and Chernin, L.S. (2005) Activity of Serratia plymuthica IC1270 gene chiA promoter region in Escherichia coli mutants deficient in global regulators of transcription. J. Basic Microbiol. 45(6): 427-439.

Madmony, A., Chernin, L., Pleban, S., Peleg, E. and Riov, J. (2005). Enterobacter cloaceae, an obligatory endophyte of pollen grains of Mediterranean pines. Folia Microbiol. 50: 209-216.,

Meziane, H., Gavriel, S., Ismailov, Z., Chet, I., Chernin, L. and Höfte, M. (2006). Control of green and blue mould on orange fruits by Serratia plymuthica strains IC14 and IC1270 and putative modes of action. Postharvest Biol. Technol. 39: 125-133.,

Veselova M.A., Lipasova V.A., Astaurova O.B., Atamova E.E., Protsenko M.A., Buza N.L., Metlitskaya A.Z., Danilova N.N., Chernin L.S. and Khmel I.A. (2006) Quorum-Sensing regulation in soil pseudomonads. Microbiology 75: 398–400.

Liu, X., Bimerew, M., Ma, Y., Müller, H., Ovadis, M., Eberl, L., Berg, G. and Chernin, L. (2007) Quorum-sensing signaling is required for production of the antibiotic pyrrolnitrin in a rhizospheric biocontrol strain of Serratia plymuthica. FEMS Microbiol. Lett. 270: 299-305.

Shedova, E., Lipasova, V.,, Velikodvorskaya, G., Ovadis, M., Chernin, L. and Khmel, I (2008) Phytase activity and its regulation in a rhizospheric strain of Serratia plymuthica. Folia Microbiol. 53: 110-114.

Veselova, M.A., Klein, Sh., Bass, I.A., Lipasova , V.A., Metlitskaya, A.Z., Ovadis, M.I., Chernin, L.S. and Khmel, I.A. (2008) Quorum Sensing Systems of Regulation,Synthesis of Phenazine Antibiotics, and Antifungal Activity in Rhizospheric Bacterium Pseudomonas chlororaphis 449. Rus. J. Genet. 44: 1400–1408.

Mueller, H., Westendorf, C., Leitner, E., Chernin, L., Riedel, K., Eberl, L., and Berg, G. (2009) Quorum sensing effects in the antagonistic rhizosphere bacterium Serratia plymuthica HRO-C48. FEMS Microbiol. Ecol. 67: 468 - 478.

Pang, Y., Liu, X., Ma, Y., Chernin, L., Berg, G. and Gao, K. (2009) Induction of systemic resistance, root colonization and biocontrol activities of rhizospheric strain of Serratia plymuthica are dependent on N-acyl homoserine lactone. Eur. J. Plant Pathol. 124: 261-268.

De Vleesschauwer, D., Chernin, L. and Hofte, M. (2009) Differential effectiveness of Serratia plymuthicaIC1270-induced systemic resistance against hemibiotrophic and necrotrophic leaf pathogens in rice. BioMed Central (BMC) Plant Biology, 9, 9.

Zaitseva, J. V., Voloshina, P. V., Liu, X., Ovadis, M. I., Berg, G., Chernin, L. S. and Khmel, I. A. (2010)Involvement of the global regulators GrrS, RpoS, and SplIR in formation of biofilms in Serratia plymuthica., Rus. J. Genet. ,46: 541-545.

Viterbo A, Landau U, Kim S, Chernin L, Chet I (2010) Characterization of ACC deaminase from the biocontrol and plant growth promoting agent Trichoderma asperellum T203. FEMS Microbiol. Lett. 305: 42-48.,

Toklikishvili, N., Dandurishvili, N., Vainstein, A., Tediashvili, M., Giorgobiani, N., Lurie, S., Szegedi, E., Glick, B.R., and L. Chernin (2010), ACC deaminase-producing bacteria inhibit crown gall formation in tomato plants infected by Agrobacterium tumefaciens or A. vitis. Plant Pathol. 59: 1023-1030.

Dandurishvili, N., Toklikishvili, N., Ovadis, M., Eliashvili, P., Giorgobiani, N., Keshelava, R., Tediashvili, M., Vainstein, A., Khmel, I., Szegedi, E., and Chernin, L. (2011) Broad-range antagonistic rhizobacteria Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown-gall tumors on tomato plants. J. Applied Microbiol., 110: 341-352.

Chernin, L., Toklikishvili, N., Ovadis, M., Kim, S., Ben-Ari, J., Khmel, I. and Vainstein, A. (2011), Quorum-sensing quenching by rhizobacterial volatiles. Environ. Microbiol. Reps. 3: 698–704.

Mozes-Koch, R., Gover, O., Tanne, E., Peretz, Y., Maori, E., Chernin, L., and Sela, I. (2012). Expression of an Entire Bacterial Operon in Plants. Plant Physiol. 158: 1883–1892.

Popova, A.A., Koksharova, O.A., Lipasova, V.A., Zaitseva,  J.V.Katkova-Zhukotskaya, O.A.Eremina, S.I.,Mironov, A.S.Chernin, L.S., and  Khmel I.A. (2014) Inhibitory and toxic effects of volatiles emitted by strains of  Pseudomonas and Serratia on growth and survival of selected microorganisms, Caenorhabditis elegans, and  Drosophila melanogaster.  BioMed Research International, vol. 2014, Article ID 125704,doi:10.1155/2014/125704

Kviatkovski, I., Chernin, L., Yarnitzky, T., Frumin, I., Sobel N. and Y. Helman, Y.  (2015) Pseudomonas aeruginosa activates the quorum sensing LuxR response regulator through secretion of 2-aminoacetophenone.Chem. Commun.51, 3258-3261.

Li, J., Yang, Y., Dubern, J.-F., Li, H., Halliday, N., Chernin, L., Gao, K., Cámara, M., and Liu, X. (2015) Regulation of GacA in Pseudomonas chlororaphis strains shows a niche specificity. PLoS ONE 10(9): e0137553. doi:10.1371/journal.pone.0137553 .

See also: Leonid Chernin



Growth enhancement of plants 
(US Patent US20140325707 A1, EP 2761007 A1) 
by I. Sela, H. D. Rabinowitch, L. Chernin, R. Mozes-Koch.

A method and kit for detection of Pseudomonas aeruginosa 
(US Patent Application No. 61/947,080) 
by Y. Helman, N. Sobel, S. Shushan, I. Kviatkovski, I. Frumin, L. Chernin.

See also: Leonid Chernin