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A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

Citation:

Kviatkovski, I. ; Yarnitzky, T. ; Shushan, S. ; Schwartz-Harari, O. ; Nir-Paz, R. ; Helman, Y. . A Bacterial Biosensor Encoding A Genetically Modified Luxr Receptor Exhibits Improved Detection Of Pseudomonas Aeruginosa'S Biomarker Molecule 2-Aminoacetophenone. Chemical Communications 2018, 54, 9218 - 9221.

Date Published:

2018

Abstract:

2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.

Publisher's Version

Last updated on 12/26/2019