Publications by Year

<embed>

Publications by Authors

5efb8d67c70d1612406abcdcfac78a61

Recent Publications

Contact Us

Department of Plant Pathology and Microbiology
The Robert H. Smith Faculty of Agriculture, Food & Environment
The Hebrew University of Jerusalem

P.O. Box 12 
Rehovot 76100 
ISRAEL

Tel: 08-9489219
Fax: 08-9466794
rakefetk@savion.huji.ac.il

Inactivation of Listeria monocytogenes on paperboard, a food packaging material, using 410 nm light emitting diodes

Citation:

Ghate, V. ; Zelinger, E. ; Shoyhet, H. ; Hayouka, Z. Inactivation of Listeria monocytogenes on paperboard, a food packaging material, using 410 nm light emitting diodes. Food Control 2019, 96, 281-290.

Abstract:

Light emitting diodes of wavelength 410 nm were used to inactivate Listeria monocytogenes stains on paperboard, an increasingly popular food packaging material. The integrity of the cell membranes was examined using differential fluorescent staining. Scanning electron microscopy (SEM) was used to obtain a deeper understanding of L. monocytogenes stain formation on paperboard and the damage caused to the cells by the LEDs. While the planktonic L. monocytogenes population could be completely inactivated following a brief lag phase that lasted about 20 min, the illumination of the sessile population left some persisters despite immediate commencement of the inactivation. Planktonic populations of inocula sized 3, 5 and 7 log CFU/mL were reduced below the detection limit in 54, 80 and 84 min respectively, whereas it took 120 and 390 min to reach constancy in the sessile populations of inocula sized 5 and 7 log CFU/cm2. The number of membrane-damaged cells was seen to increase with the illumination time. SEM images provided evidence of the protection conferred by the stain on the underlying cells. This study demonstrates that blue LEDs have the potential to reduce the risk of L. monocytogenes contamination from paperboard cartons under refrigeration. © 2018 Elsevier Ltd

Website