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Department of Plant Pathology and Microbiology
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The Hebrew University of Jerusalem

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In Planta Colonization and Role of T6SS in Two Rice Kosakonia Endophytes

Citation:

Mosquito, S. ; Bertani, I. ; Licastro, D. ; Compant, S. ; Myers, M. P. ; Hinarejos, E. ; Levy, A. ; Venturi, V. In Planta Colonization and Role of T6SS in Two Rice Kosakonia Endophytes. Molecular Plant-Microbe Interactions® 2019, MPMI-09-19-0256-R.

Abstract:

Endophytes live inside plants and are often beneficial. Kosakonia is a novel bacterial genus that includes many diazotrophic plant-associated isolates. Plant–bacteria studies on two rice endophytic Kosakonia beneficial strains were performed, including comparative genomics, secretome profiling, in planta tests, and a field release trial. The strains are efficient rhizoplane and root endosphere colonizers and localized in the root cortex. Secretomics revealed 144 putative secreted proteins, including type VI secretory system (T6SS) proteins. A Kosakonia T6SS genomic knock-out mutant showed a significant decrease in rhizoplane and endosphere colonization ability. A field trial using rice seed inoculated with Kosakonia spp. showed no effect on plant growth promotion upon nitrogen stress and microbiome studies revealed that Kosakonia spp. were significantly more present in the inoculated rice. Comparative genomics indicated that several protein domains were enriched in plant-associated Kosakonia spp. This study highlights that Kosakonia is an important, recently classified genus involved in plant–bacteria interaction.

Notes:

PMID: 31609645

Publisher's Version

Last updated on 01/12/2020